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[subject] => 抗体药物研发流程图操作大鼠ELISA试剂盒过程中需要注意的事情
[dateline] => 2024-3-14 16:04:18
[message] => 1. 大鼠ELISA试剂盒试剂的选择
通常所使用的ELISA试剂盒或其它试剂，必须注册批准、批检合格、抗体药物研发流程图临床评估质量优良，并且均在有效期内使用。不同批号试剂组分不得混用，过期的试剂一律不得使用，首先提升实验结果的可信度。
2. 大鼠ELISA试剂盒样品的采集与保存
用于检测的标本应保持新鲜，标本的采集应尽量避免溶血，因红细胞破裂可释放过氧化物酶活性物质，干扰结果，会出现假阳性;标本如果受细菌污染，会因菌体中含有内源性过氧化物酶而出现假阳性; 混浊或有沉淀的血清样品应离心，待澄清后方可检测，避免对结果造成影响。
用于HIV抗体检测的血清或血浆样品应存放于-20°℃以下，短期(1周内)进行检测的样品可存放于2°℃~8℃。融解时应上下颠倒充分混匀，动作要轻缓，避免产生气泡。同时应注意避免样品反复冻融，否则会造成样品抗体效价降低，影响其结果。
3. 大鼠ELISA试剂盒加样
加样前应注意将微孔板条从冷藏环境中取出放置室温，待试剂平衡后方可使用，未用完者需放入有干燥剂的密封袋中保存。整个HIV抗体检测必须符合《全国艾滋病检测技术规范》，严格防止交叉感染。操作时必须戴手套、穿工作衣，严格执行消毒隔离制度。
大鼠ELISA试剂盒
加样要用微量移液器(要求准确性高并定期进行校正)，按规定的量加入孔内，避免加在孔壁的边缘，垂直加样，不要使吸头碰到酶标板孔上的已包被的抗原，加样时尽量慢吸快放，同时注意不要溅出，避免产生气泡。
每次加样都要更换吸头，做到一样一吸头，防止发生交叉污染，干吸头预先在血清中抽吸3次，以便保证加样量的准确性。滴加试剂应先将滴瓶摇匀后再进行滴加，以免产生气泡。
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